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Topiramate ameliorates abdominal aorta cross-clamping induced liver injury in rats
Erkan Cure1, Medine C Cure2, Levent Tumkaya3, Yildiray Kalkan3, Ibrahim Aydin4, Aynur Kirbas2, Arif Yilmaz5, Suleyman Yuce1, Mehmet F Gokce6
1 Department of Internal Medicine, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey
2 Department of Biochemistry, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey
3 Department of Histology and Embryology, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey
4 Department of Surgery, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey
5 Department of Gastroenterology, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey
6 Department of Physiology, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey
Correspondence Address:
Dr. Erkan Cure
Department of Internal Medicine, University of Recep Tayyip Erdogan, School of Medicine, Rize - 53100
Turkey
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/1319-3767.141690
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Background and Aim : Ischemia/reperfusion (I/R) injury in the liver occurs after a prolonged period of ischemia followed by restoration of hepatic blood perfusion. During the surgery of abdominal aorta, I/R injury causes damage to lower extremities and many organs, especially liver. The antioxidant and tumor necrosis factor-alpha (TNF-α) suppression effects of topiramate (TPM) have been reported in several studies. We evaluated the potential protective effect of TPM on cellular damage in liver tissue during I/R injury. Materials and Methods : Thirty male Wistar albino rats were divided into three groups: Control, I/R, and I/R plus TPM (I/R + TPM) groups. Laparotomy without I/R injury was performed in the control group. After laparotomy, cross-ligation of infrarenal abdominal aorta was applied for 2 h in I/R groups that was followed by 2 h of reperfusion. TPM (100 mg/kg/day) was orally administrated to the animals in the I/R + TPM group for seven consecutive days before I/R procedure. Results : The I/R group's TNF-α and interleukin-6 (IL-6) levels were significantly higher than those of the control (P = 0.010; P = 0.002) and I/R + TPM groups (P = 0.010; P = 0.002, respectively). Asymmetric dimethyl arginine (ADMA) levels of I/R group were higher than the control (P = 0.015) and I/R + TPM groups. I/R caused serious histopathological damage to liver tissue; however, TPM led to very low histopathological changes. Conclusion : Our data demonstrated that TPM treatment prominently decreases the severity of liver I/R injury. TPM pretreatment may have preventive effects on liver injury via I/R during intra-abdominal surgery. |
