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Composition of the gut microbiota in patients with inflammatory bowel disease in Saudi Arabia: A pilot study
Hadba Al-Amrah1, Omar I Saadah2, Mahmoud Mosli3, Vito Annese4, Rashad Al-Hindi1, Sherif Edris5, Dikhnah Alshehri6, Hanan Alatawi6, Marfat Alatawy6, Ahmad Bahieldin7
1 Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
2 Department of Pediatrics, Faculty of Medicine; Department of Inflammatory Bowel Disease Research Group, King Abdulaziz University, Jeddah, Saudi Arabia
3 Department of Inflammatory Bowel Disease Research Group; Department of Internal Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
4 Fakeeh University Hospital, Dubai, United Arab Emirates
5 Department of Biological Sciences, Faculty of Science, King Abdulaziz University; Princess Al Jawhara Albrahim Centre of Excellence in Research of Hereditary Disorders (PACER-HD), King Abdulaziz University, Jeddah, Saudi Arabia; Department of Genetics, Ain Shams University, Cairo, Egypt; Al Borg Medical Laboratories, Al Borg Diagnostics, Jeddah, Saudi Arabia
6 Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah; Department of Biological Sciences, College of Science, Univesity of Tabuk, Tabuk, Saudi Arabia
7 Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Genetics, Ain Shams University, Cairo, Egypt
Correspondence Address:
Omar I Saadah,
Inflammatory Bowel Disease Research Group, King Abdulaziz University, Jeddah
Saudi Arabia
 Source of Support: None, Conflict of Interest: None DOI: 10.4103/sjg.sjg_368_22
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Conclusions: The results of this study provide an overview of the variations in microbiota diversity present in Saudi IBD patients compared to healthy controls.
Results: The key finding was three negative bacterial biomarkers, Paraprevotellaceae, the Muribaculaceae families of Bacteroidetes phylum, and the Leuconostocaceae family of Firmicutes phylum, which had a higher relative abundance in healthy individuals compared to IBD patients. It was also found that primary microbiota signatures at certain genera and species levels, including Prevotella copri, Bifidobacterium adolescentis, Ruminococcus callidus, Coprococcus sp., Ruminococcus gnavus, Dorea formicigenerans, Leuconostoc, Dialister, Catenibacterium, Eubacterium biforme, and Lactobacillus mucosae, were absent in almost all IBD patients, while Veillonella dispar was absent in all healthy individuals.
Methods: After obtaining an informed consent, fecal samples were collected from 11 participants with IBD (patients) and 10 healthy individuals (controls). The bacterial components of the microbial population were identified by next-generation sequencing of partial 16S rRNA. Statistically significant dissimilarities were observed between samples for all metrics.
Background: Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory condition attributed to a complex interaction between imbalances in the gut microbiome, environmental conditions, and a deregulated immune response. The aim of the study was to investigate the composition of the gut microbiome of Saudi patients with IBD.
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