Home
About us
Instructions
Submission
Subscribe
Advertise
Contact
Reader Login
Current Issue
Past Issues
Ahead of Print
Search
Users Online:
639
Export selected to
Endnote
Reference Manager
Procite
Medlars Format
RefWorks Format
BibTex Format
Citation statistics : Table of Contents
2013| January-February | Volume 19 | Issue 1
Online since
January 11, 2013
Archives
Previous Issue
Next Issue
Most popular articles
Most cited articles
Show all abstracts
Show selected abstracts
Export selected to
Cited
Viewed
PDF
REVIEW ARTICLE
The importance of interstitial cells of cajal in the gastrointestinal tract
Othman A Al-Shboul
January-February 2013, 19(1):3-15
DOI
:10.4103/1319-3767.105909
PMID
:23319032
Gastrointestinal (GI) motility function and its regulation is a complex process involving collaboration and communication of multiple cell types such as enteric neurons, interstitial cells of Cajal (ICC), and smooth muscle cells. Recent advances in GI research made a better understanding of ICC function and their role in the GI tract, and studies based on different types of techniques have shown that ICC, as an integral part of the GI neuromuscular apparatus, transduce inputs from enteric motor neurons, generate intrinsic electrical rhythmicity in phasic smooth muscles, and have a mechanical sensation ability. Absence or improper function of these cells has been linked to some GI tract disorders. This paper provides a general overview of ICC; their discovery, subtypes, function, locations in the GI tract, and some disorders associated with their loss or disease, and highlights some controversial issues with regard to the importance of ICC in the GI tract.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
39
22,584
3,164
ORIGINAL ARTICLES
p53 and beta-catenin expression in gallbladder tissues and correlation with tumor progression in gallbladder cancer
Mila Ghosh, Puja Sakhuja, Shivendra Singh, Anil K Agarwal
January-February 2013, 19(1):34-39
DOI
:10.4103/1319-3767.105922
PMID
:23319036
Background/Aim:
The inactivation of the tumor suppressor gene and activation of the proto-oncogene are key steps in the development of human cancer. p53 and beta-catenin are examples of such genes, respectively. In the present study, our aim was to determine the role of these genes in the carcinogenesis of the gallbladder by immunohistochemistry.
Patients and Methods:
Sections from paraffin-embedded blocks of surgically resected specimens of gallbladder cancer (GBC) (80 cases), chronic cholecystitis (60 cases), and control gallbladders (10 cases) were stained with the monoclonal antibody p53, and polyclonal antibody beta-catenin. Results were scored semiquantitatively and statistical analysis performed. p53 expression was scored as percentage of the nuclei stained. Beta-catenin expression was scored as type of expression-membranous, cytoplasmic, and nuclear staining. Beta-catenin expression was correlated with tumor invasiveness, differentiation, and stage.
Results:
Over-expression of p53 was seen in 56.25% of GBC cases and was not seen in chronic cholecystitis or in control gallbladders. p53 expression in gallbladder cancer was significantly higher than in inflammatory or control gallbladders (
P
< 0.0001). p53 expression increased with increasing tumor grade (
P
= 0.039). Beta-catenin nuclear expression was seen in 75% cases of gallbladder cancer and in no case of chronic cholecystitis and control gallbladder. Beta-catenin nuclear expression increased with tumor depth invasiveness, and grade (
P
= 0.028 and
P
= 0.0152, respectively).
Conclusion:
p53 and beta-catenin nuclear expression is significantly higher in GBC. p53 expression correlates with increasing tumor grade while beta-catenin nuclear expression correlates with tumor grade and depth of invasion, thus suggesting a role for these genes in tumor progression of GBC.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
8
3,728
556
Inhibitory control test, critical flicker frequency, and psychometric tests in the diagnosis of minimal hepatic encephalopathy in cirrhosis
Praveen Sharma, Ajay Kumar, Shweta Singh, Pankaj Tyagi, Ashish Kumar
January-February 2013, 19(1):40-44
DOI
:10.4103/1319-3767.105924
PMID
:23319037
Background/Aim:
Minimal hepatic encephalopathy (MHE) impairs health-related quality of life and driving ability of the patient.
Objective:
We assessed the utility of the inhibitory control test (ICT), critical flicker frequency (CFF), and psychometry in the diagnosis of MHE.
Patients and Methods:
Consecutive patients with cirrhosis underwent number connection tests A and B (NCT-A, B), digit symbol test (DST), line tracing test (LTT), serial dot test (SDT), CFF, and ICT at baseline and after four hours. Fifty healthy subjects served as controls for the ICT test.
Results:
Fifty patients with cirrhosis (43.4 ± 10.2 yrs, M: F 42:8) underwent psychometric tests [NCT-A (48.3 ± 17.7 vs. 42.6 ± 17.3 sec,
P
= 0.001), NCT-B (85.7 ± 40.1 vs. 90.2 ± 37.0 sec,
P
= 0.18), DST (23.5 ± 9.3 vs. 23.0 ± 8.7,
P
= 0.45), LTT (96.6 ± 48.2 vs. 96.8 ± 46.8 sec,
P
= 0.92), SDT (88.0 ± 39.5 vs. 83.4 ± 37.2 sec,
P
= 0.02)] at baseline and after four hours. Target accuracy of ICT was lower in patients with cirrhosis compared with controls (88.4 ± 5.6 vs. 95.6 ± 2.1,
P
= 0.01), whereas ICT lures were higher (18.3 ± 4.2 vs 10.2 ± 2.8,
P
= 0.01). Patients with cirrhosis showed a reduction in lures in the second evaluation compared with the first (18.3 ± 4.2 vs. 17.1 ± 4.3,
P
= 0.003) but no change in target accuracy (88.4 ± 5.6 vs. 88.4 ± 5.3,
P
= 0.97). Control subjects did not show any change either in lures (10.2 ± 2.8 vs. 10.3 ± 2.1,
P
= 0.65) or target accuracy (95.6 ± 2.1 vs. 95.5 ± 2.2,
P
= 0.82). The sensitivity and specificity of ICT test for the diagnosis of MHE at lure rate >16.5 was 88.5 and 56%, respectively. CFF in patients with MHE (38.4 ± 1.8 vs. 38.6 ± 1.5,
P
= 0.3) and non MHE (40.6 ± 2.2 vs. 40.8 ± 2.2,
P
= 0.6) did not show any difference after four hours as in controls (41.9 ± 2.4 vs. 42.1 ± 2.0,
P
= 0.3). Thirty one (31%) patients preferred psychometric tests, 57 (57%) preferred CFF and only 12 (12%) preferred ICT (
P
= 0.001).
Conclusions:
ICT, CFF, and psychometric tests are useful tools to assess MHE, and CFF was preferred by this study cohort.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
8
4,668
664
Hepatitis C genotype 4: Genotypic diversity, epidemiological profile, and clinical relevance of subtypes in Saudi Arabia
Hamad I Al Ashgar, Mohammed Q Khan, Mohammed Al-Ahdal, Sahar Al Thawadi, Ahmad Salem Helmy, Ahmed Al Qahtani, Faisal M Sanai
January-February 2013, 19(1):28-33
DOI
:10.4103/1319-3767.105920
PMID
:23319035
Background/Aim:
Hepatitis C virus genotypes 4 (HCV-4) is the most prevalent genotype in Saudi Arabia, although it's various subtypes, mode and route of transmission remains unknown. The aim of this study was to analyze (i) the variability of the HCV-4 subtypes, the route and source of HCV transmission and (ii) the influence of HCV-4 subtypes on their therapeutic response.
Patients and
Methods
: Sixty-four HCV-4 patients were analyzed retrospectively for the prevalence of various sub-genotypes and the possible mode of transmission, and it was correlated with their treatment response to pegylated interferon (PEG-IFN) α-2a and ribavirin therapy.
Results:
Positive history of blood or blood products transfusion was noted in 22 patients (34%), hemodialysis in 10 patients (15.6%), surgery in 7 patients (11%), and unknown etiology in 25 patients (39%). Prevalence of HCV-4 subtypes was 4a = 48.4% (31/64), 4d = 39% (25/64), 4n = 6.25% (4/64), and remaining combined (4m, 4l, 4r, 4o) 6.25% (4/64). No significant correlation between subtypes and the source of transmission was recognized (
P
= 0.62). Sustained virological response in all HCV-4 patients was 64% (41/64), while in each subtypes separately it was 4a 77.4% (24/31), 4d 52% (13/25), and combined (4n, 4m, 4l, 4r, 4o) 62.5% (5/8) (
P
= 0.046).
Conclusion:
No obvious cause for the mode of HCV transmission was noted in majority of the patients. No significant correlation was observed between HCV-4 subtypes and the source of HCV infection. 4a and 4d subtypes were the most common in Saudi Arabia, and patients infected with 4a subtype responded significantly better to combination therapy than to 4d subtype.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
7
4,518
522
Pancreatic cystic neoplasms: Predictors of malignant behavior and management
Ehab Atef, Ayman El Nakeeb, Ehab El Hanafy, Mohamed El Hemaly, Emad Hamdy, Ahmed El-Geidie
January-February 2013, 19(1):45-53
DOI
:10.4103/1319-3767.105927
PMID
:23319038
Background/Aim:
Pancreatic cystic neoplasms are being increasingly identified with the widespread use of advanced imaging techniques. In the absence of a good radiologic or pathologic test to preoperatively determine the dianosis, clinical characteristics might be helpful. The objectives of this analysis were to define the incidence and predictors of malignancy in pancreatic cysts.
Patients and Methods:
Patients with true pancreatic cysts who were treated at our institution were included. Patients with documented pseudocysts were excluded. Demographic data, clinical manifestations, radiological, surgical, and pathological records of those patients were reviewed.
Results:
Eighty-one patients had true pancreatic cyst. The mean age was 47 ± 15.5 years. There were 28.4% serous cystadenoma, 21% mucinous cystadenoma, 6.2% intraductal papillary tumors, 8.6% solid pseudopapillary tumors, 1.2% neuroendocrinal tumor, 3.7% ductal adenocarcinoma, and 30.9% mucinous cystadenocarcinoma. Malignancy was significantly associated with men (
P
= 0.04), older age (0.0001), cysts larger than 3 cm in diameter (
P
= 0.001), presence of solid component (
P
= 0.0001), and cyst wall thickening (
P
= 0.0001). The majority of patients with malignancy were symptomatic (26/28, 92.9%). The symptoms that correlated with malignancy included abdominal pain (
P
= 0.04) and weight loss (
P
= 0.0001). Surgical procedures were based on the location and extension of the lesion.
Conclusion:
The most common pancreatic cysts were serous and mucinous cysts. These tumors were more common in females. Old age, male gender, large tumor, presence of solid component, wall thickness, and presence of symptoms may predict malignancy in the cyst.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
5
7,887
816
Emerging inflammatory bowel disease in saudi outpatients: A report of 693 cases
Mohammad A Al-Mofarreh, Ibrahim A Al-Mofleh
January-February 2013, 19(1):16-22
PMID
:23319033
Background/Aim:
Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology and considered traditionally as a disease of the western world. Recently, rising trends have been observed in countries previously known to have a low prevalence and incidence. The aim of this study is to collect epidemiological data on IBD outpatients and to add data from the Kingdom of Saudi Arabia (KSA) to the available IBD literature.
Patients and Methods:
The medical records of 693 Saudi patients with IBD over a period of 17 years, between 1993 and 2009, were reviewed. The demographic and clinical data and methods of diagnosis were retrieved.
Results:
The total number of patients in this cohort was 693. It constituted 238 (34.3%) ulcerative colitis (UC) and 455 (65.7%) Crohn's disease (CD) patients. UC was steady throughout the years, whereas only 1.2 CD patients were diagnosed per year in the first 11 years, and 73.7 per year in the last six years. The median age of UC patients was 34 years, ranging from 10 to 80 years with a peak between 21 and 40 years and in CD it was 27 years, ranging from 11 to 73 years with a peak between 11 and 30 years. There was a male preponderance of 1.5:1 and 2:1, respectively. The rest of the data is discussed in this study.
Conclusion:
IBD is no longer a rare disease in KSA. UC is in a steady state, whereas CD is increasing significantly and far outnumbering UC.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
4
7,416
674
Immunohistochemical expression of matrix metalloproteinase-7 in human colorectal adenomas using specified automated cellular image analysis system: A clinicopathological study
Ban J Qasim, Hussam H Ali, Alaa G Hussein
January-February 2013, 19(1):23-27
DOI
:10.4103/1319-3767.105916
PMID
:23319034
Background/Aim:
To evaluate the immunohistochemical expression of matrix metalloproteinase-7 (MMP-7) in colorectal adenomas, and to correlate this expression with different clinicopathological parameters.
Patients and Methods:
The study was retrospectively designed. Thirty three paraffin blocks from patients with colorectal adenoma and 20 samples of non-tumerous colonic tissue taken as control group were included in the study. MMP-7 expression was assessed by immunohistochemistry method. The scoring of immunohistochemical staining was conducted utilizing a specified automated cellular image analysis system (Digimizer).
Results:
The frequency of positive immunohistochemical expression of MMP-7 was significantly higher in adenoma than control group (45.45% versus 10%) (
P
value < 0.001). Strong MMP-7 staining was mainly seen in adenoma cases (30.30%) in comparison with control (0%) the difference is significant (
P
< 0.001). The three digital parameters of MMP-7 immunohistochemical expression (Area (A), Number of objects (N), and intensity (I)) were significantly higher in adenoma than control. Mean (A and I) of MMP-7 showed a significant correlation with large sized adenoma (≥ 1cm) (
P
< 0.05), also a significant positive correlation of the three digital parameters (A, N, and I) of MMP-7 expression with villous configuration and severe dysplasia in colorectal adenoma had been identified (
P
< 0.05).
Conclusion:
MMP-7 plays an important role in the growth and malignant conversion of colorectal adenomas as it is more likely to be expressed in advanced colorectal adenomatous polyps with large size, severe dysplasia and villous histology. The use of automated cellular image analysis system (Digmizer) to quantify immunohistochemical staining yields more consistent assay results, converts semi-quantitative assay to a truly quantitative assay, and improves assay objectivity and reproducibility.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
3
3,211
410
EDITORIAL
Inflammatory bowel disease: A global disease
Subrata Ghosh, Majid A Almadi
January-February 2013, 19(1):1-2
DOI
:10.4103/1319-3767.105905
PMID
:23319031
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
[PubMed]
2
3,674
674
IMAGE QUIZ
Colonic lacerations, mucosal scars and image enhancement: An on-the-spot diagnosis
Alexandros Smirnidis, Efstratios Alexandridis, Anastasios Koulaouzidis
January-February 2013, 19(1):54-55
DOI
:10.4103/1319-3767.105929
PMID
:23319039
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[PubMed]
-
2,577
289
LETTER TO EDITOR
Rifaximin as treatment for hepatic encephalopathy: Some considerations
Lorenzo Ridola, Angelo Zullo, Cesare Hassan
January-February 2013, 19(1):56-56
DOI
:10.4103/1319-3767.105930
PMID
:23319040
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[PubMed]
-
2,943
526
Search
The Journal
Site Statistics
Addresses
My Preferences
Online Submission
About us
|
Contact us
|
Sitemap
|
Advertise
|
What's New
|
Copyright and Disclaimer
|
Privacy Notice
© Saudi Journal of Gastroenterology (Official journal of The Saudi Gastroenterology Association) | Published by Wolters Kluwer -
Medknow
Online since 15
th
October, 2006